Journal: The Journal of comparative neurology

Article Title: The distribution, number and certain neurochemical identities of infracortical white matter neurons in a lar gibbon ( Hylobates lar ) brain.

doi: 10.1002/cne.24545

Figure Lengend Snippet: Sources and dilution of antibodies used in the current study.

Article Snippet: The antibodies used and associated details are listed in . table ft1 table-wrap mode="anchored" t5 Table 1: caption a7 Antibody Host Immunogen Manufacturer Catalogue No. Reference Dilution RRID NeuN Rabbit GST-tagged recombinant protein corresponding to mouse NeuN Merck-Millipore ABN78C3 Ngwenya et al., 2016 1:500 AB_11204707 PV rabbit Rat muscle parvalbumin Swant PV28 Hirano et al., 2011 1:10000 AB_10000343 CB rabbit Rat recombinant calbindin D-28k Swant CB38a Bunce et al., 2013 1:10000 AB_10000340 CR rabbit Recombinant human calretinin containing a 6-his tag at the N-terminal Swant 7699/3H Adrio et al., 2011 1:10000 AB_10000321 nNOS rabbit Recombinant human neuronal nitric oxide synthase Merck-Millipore AB5380 Russo et al., 2013 1:6000 AB_91824 Open in a separate window Sources and dilution of antibodies used in the current study.

Techniques: Recombinant

Journal: The Journal of comparative neurology

Article Title: The distribution, number and certain neurochemical identities of infracortical white matter neurons in a lar gibbon ( Hylobates lar ) brain.

doi: 10.1002/cne.24545

Figure Lengend Snippet: Stereological parameters used for estimating neuronal numbers in the white matter of the lar gibbon. NeuN – neuronal nuclear marker; nNos – neuronal nitric oxide synthase; CR – calretinin.

Article Snippet: The antibodies used and associated details are listed in . table ft1 table-wrap mode="anchored" t5 Table 1: caption a7 Antibody Host Immunogen Manufacturer Catalogue No. Reference Dilution RRID NeuN Rabbit GST-tagged recombinant protein corresponding to mouse NeuN Merck-Millipore ABN78C3 Ngwenya et al., 2016 1:500 AB_11204707 PV rabbit Rat muscle parvalbumin Swant PV28 Hirano et al., 2011 1:10000 AB_10000343 CB rabbit Rat recombinant calbindin D-28k Swant CB38a Bunce et al., 2013 1:10000 AB_10000340 CR rabbit Recombinant human calretinin containing a 6-his tag at the N-terminal Swant 7699/3H Adrio et al., 2011 1:10000 AB_10000321 nNOS rabbit Recombinant human neuronal nitric oxide synthase Merck-Millipore AB5380 Russo et al., 2013 1:6000 AB_91824 Open in a separate window Sources and dilution of antibodies used in the current study.

Techniques: Marker, Sampling

Journal: The Journal of comparative neurology

Article Title: The distribution, number and certain neurochemical identities of infracortical white matter neurons in a lar gibbon ( Hylobates lar ) brain.

doi: 10.1002/cne.24545

Figure Lengend Snippet: Frequency distribution bar plots of somal volumes of WMICs in the brain of the lar gibbon. (a) Volumes of the soma of WMICs immunoreactive to neuronal nuclear marker (NeuN). Note the median volume is 615.9 μm3, with a range from 63.6 to 3 716.6 μm3. (b) Volumes of the soma of WMICs immunoreactive to neuronal nitric oxide synthase (nNOS). Note the median volume is 817.8 μm3, with a range from 110.6 to 2 899.8 μm3. (c) Volumes of the soma of WMICs immunoreactive to calretinin. Note the median volume is 619.4 μm3, with a range from 56.2 to 1 952.7 μm3. In all plots the bin width = 92.9 μm3.

Article Snippet: The antibodies used and associated details are listed in . table ft1 table-wrap mode="anchored" t5 Table 1: caption a7 Antibody Host Immunogen Manufacturer Catalogue No. Reference Dilution RRID NeuN Rabbit GST-tagged recombinant protein corresponding to mouse NeuN Merck-Millipore ABN78C3 Ngwenya et al., 2016 1:500 AB_11204707 PV rabbit Rat muscle parvalbumin Swant PV28 Hirano et al., 2011 1:10000 AB_10000343 CB rabbit Rat recombinant calbindin D-28k Swant CB38a Bunce et al., 2013 1:10000 AB_10000340 CR rabbit Recombinant human calretinin containing a 6-his tag at the N-terminal Swant 7699/3H Adrio et al., 2011 1:10000 AB_10000321 nNOS rabbit Recombinant human neuronal nitric oxide synthase Merck-Millipore AB5380 Russo et al., 2013 1:6000 AB_91824 Open in a separate window Sources and dilution of antibodies used in the current study.

Techniques: Marker

Journal: The Journal of comparative neurology

Article Title: The distribution, number and certain neurochemical identities of infracortical white matter neurons in a lar gibbon ( Hylobates lar ) brain.

doi: 10.1002/cne.24545

Figure Lengend Snippet: Photomicrographs of neuronal nitric oxide synthase (nNOS) immunostaining in the rostral portion of the frontal lobe of the lar gibbon showing the distribution of WMICs immunoreactive to nNOS. (a) Moderately magnified image of the superior frontal gyrus (from the region indicated by the b in Fig. 1a), showing the numerous nNOS-immunoreactive WMICs deep to the cerebral cortex (grey matter, GM, white matter, WM). The approximate boundary of the deep border of cortical layer VI and the WM is marked by a dashed line. (b) High magnification image of the cortical/white matter boundary (marked by a dashed line) of the superior frontal gyrus, showing the nNOS-immunoreactive WMICs deep to the cerebral cortex. Arrows in a and b indicate the same neuron for orientation of image location. (c) Moderately magnified image of the fundus of the inferior frontal gyrus (from the region indicated by the d in Fig. 1a), showing the nNOS-immunoreactive WMICs deep to the cerebral cortex (GM) within the WM. The approximate boundary of the deep border of cortical layer VI and the WM is marked by a dashed line. (d) High magnification image of the cortical/white matter boundary (marked by a dashed line) of the fundus of the inferior frontal gyrus, showing the nNOS-immunoreactive WMICs deep to the cerebral cortex within the WM. Arrows in c and d indicate the same neuron for orientation of image location. Scale bar in c = 500 μm and apply to a and c. Scale bar in d = 250 μm and applies to b and d. In all images dorsal is to the top of the image and medial to the left.

Article Snippet: The antibodies used and associated details are listed in . table ft1 table-wrap mode="anchored" t5 Table 1: caption a7 Antibody Host Immunogen Manufacturer Catalogue No. Reference Dilution RRID NeuN Rabbit GST-tagged recombinant protein corresponding to mouse NeuN Merck-Millipore ABN78C3 Ngwenya et al., 2016 1:500 AB_11204707 PV rabbit Rat muscle parvalbumin Swant PV28 Hirano et al., 2011 1:10000 AB_10000343 CB rabbit Rat recombinant calbindin D-28k Swant CB38a Bunce et al., 2013 1:10000 AB_10000340 CR rabbit Recombinant human calretinin containing a 6-his tag at the N-terminal Swant 7699/3H Adrio et al., 2011 1:10000 AB_10000321 nNOS rabbit Recombinant human neuronal nitric oxide synthase Merck-Millipore AB5380 Russo et al., 2013 1:6000 AB_91824 Open in a separate window Sources and dilution of antibodies used in the current study.

Techniques: Immunostaining